Bacterial Communities of Frozen Quaternary Sediments of Marine Origin on the Coast of Western Spitsbergen

The bacterial composition of permafrost samples taken during drilling frozen marine sediments in the area Barentsburg coal mine on east coast Grønfjord Bay Western Spitsbergen has been studied. study was based analysis V4 region 16S rRNA gene, carried out using next generation sequencing, as well classical microbiological methods (direct luminescence microscopy and aerobic cultivation).The total cell number ranges from 6.73 ± 0.73 × 106 to 3.37 0.19 107 cells per g. cultivable bacteria 1/5 TSA R2A media 0 6.20 0.45 104 CFU/g. Isolates were identified by gene representatives genera Arthrobacter, Pseudarthrobacter, Psychrobacter, Rhodoferax. dominant phyla domain Bacteria Actinobacteria, Proteobacteria, Chloroflexi, Nitrospirae Firmicutes. As a result phylogenetic operational taxonomic units, methane oxidizing, sulfate reducing bacteria, heterotrophic involved transformation organic matter found.